FACScan Analysis of WBC Dilution Series from Donor Peripheral Blood Immunostained for
Chlamydia. A buffy coat pool prepared from fresh human samples was assessed as positive for Chlamydia by immunostaining. The pool was immunostained for Chlamydia, serially diluted with RBCs pooled from the same samples then analyzed with a FACScan flow cytometer. The graphed results of the series demonstrates detection of Chlamydia infected cells in this non clinical down to 0.5% WBC/sample, a level of 3 ± 1.5 infected cells in the total of 10,000 cells counted.