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Figure 3 | BMC Infectious Diseases

Figure 3

From: Detection of Chlamydia in the peripheral blood cells of normal donors using in vitroculture, immunofluorescence microscopy and flow cytometry techniques

Figure 3

Infected and uninfected WBCs dual immunostained with CD Specific monoclonal and anti- Chlamydia antibody. Representative medial confocal microscopic images from the Z-axis series of optical sections through Chlamydia-infected human PBMC cells. Immunostaining used anti-Chlamydia EB antibody and either anti-CD14(monocyte), anti-CD 16b(neutrophil), or anti-CDw125 (eosinophil/basophil) mAb as indicated. Respectively, the Merge and DIC columns show the combined FITC and TRITC images, and the distribution and morphology of the cells in the field. Note: In vivo each of the three cell types can be infected by Chlamydia and the CD14 row includes a Chlamydia infected cell (TRITC positive) that is not immunostained by the ant-CD14 monoclonal (FITC negative).

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