Figure 3From: Detection of Chlamydia in the peripheral blood cells of normal donors using in vitroculture, immunofluorescence microscopy and flow cytometry techniques Infected and uninfected WBCs dual immunostained with CD Specific monoclonal and anti- Chlamydia antibody. Representative medial confocal microscopic images from the Z-axis series of optical sections through Chlamydia-infected human PBMC cells. Immunostaining used anti-Chlamydia EB antibody and either anti-CD14(monocyte), anti-CD 16b(neutrophil), or anti-CDw125 (eosinophil/basophil) mAb as indicated. Respectively, the Merge and DIC columns show the combined FITC and TRITC images, and the distribution and morphology of the cells in the field. Note: In vivo each of the three cell types can be infected by Chlamydia and the CD14 row includes a Chlamydia infected cell (TRITC positive) that is not immunostained by the ant-CD14 monoclonal (FITC negative).Back to article page