Figure 1

Evaluation of genes differentially expressed by microarray (blue bars) with QRT-PCR (red bars). The microarray experiment was conducted as a case-control study with whole blood gene expressions given as ratio of signal intensity in each of five male infants hospitalised with respiratory syncytial virus, subtype B, bronchiolitis versus signal intensity in a corresponding one year old male control exposed to the virus during infancy but not hospitalised and/or treated for bronchiolitis. The quantitative real-time polymerase chain reaction (QRT-PCR) study used TaqMan Low Density Array (TLDA) cards with gene expression given as mean relative quantification (RQ) from triplets of each gene and analyzed in the same five male infants as selected for microarray. A pooled sample from four of the five one year old male controls was used as exogenous control and beta-glucuronidase (GUSB) was used as endogenous control in the QRT-PCR study. The following genes (gene symbol and name) were evaluated; BPGM: 2,3-bisphosphoglycerate mutase, CLC: Charcot-Leyden crystal protein, DNAPTP6: DNA polymerase-transactivated protein 6, EPSTI1: Epithelial stromal interaction 1 (breast), ERAF: Erythroid associated factor, G1P2: Interferon, alpha-inducible protein (clone IFI-15K), HBD: Hemoglobin, delta, HBE1: Hemoglobin, epsilon 1, HP: Haptoglobin, IFI27: Interferon, alpha-inducible protein 27, IFI44: Interferon-induced protein 44, IFI44L: Interferon-induced protein 44-like, MARCO: Macrophage receptor with collagenous structure, MMP9: Matrix metalloproteinase 9, MS4A4A: Membrane-spanning 4-domains, subfamily A, member 4, NQO2: NAD(P)H dehydrogenase, quinone 2, STXBP2: Syntaxin binding protein 2