Vaginal infection outcome by fluconazole-itraconazole resistant strain C. albicans (AIDS-68) in oophorectomized, oestradiol-treated rats inoculated intravaginally with TTO 5% v/v (open squares), 1% v/v (open circles), terpinen-4-ol 1% v/v (filled triangles), fluconazole 100 μg (filled circles), Tween-80 0.001% v/v (control; filled squares) at 1, 24 and 48 hr after C. albicans challenge (107 cells in 0.1 mL). Each curve represents the mean (± S.E.) of the fungal cfu of five rats. At each time point (day1-day14) there was a statistically significant difference (P < 0.05, Student's t test, two tailed) between the untreated control and cfu of rats treated with TTO 5%, TTO 1% and terpinen-4-ol. At scattered day intervals, there was also a statistically significant difference (P < 0.05, Student's t test, two tailed) between cfu of rats treated with TTO 5% or terpinen-4-ol and TTO 1% (for example day 1).