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Table 2 Retrospective Clinical HSV specimens

From: Real-Time PCR for detection of herpes simplex virus without nucleic acid extraction

Specimen Culture/IFA Real-Time PCR
     Extracteda Unextractedb
Gender Site Culture IFA Result CP value Type Result CP value Type
M rectal positive HSV-1 positive 7.09 HSV-1 positive 10.44 HSV-1
F genital positive HSV-1 positive 8.49 HSV-1 positive 11.01 HSV-1
F genital positive HSV-2 positive 12.55 HSV-2 positive 15.28 HSV-2
F genital positive HSV-2 positive 13.14 HSV-2 positive 15.32 HSV-2
M genital positive HSV-1 positive 13.14 HSV-1 positive 14.98 HSV-1
M genital positive HSV-1 positive 13.23 HSV-1 positive 15.68 HSV-1
M rectal positive HSV-1 positive 13.27 HSV-1 positive 14.51 HSV-1
M genital positive HSV-2 positive 13.70 HSV-2 positive 15.98 HSV-2
M rash/lesion positive HSV-1 positive 13.73 HSV-1 positive 15.82 HSV-1
F genital positive HSV-1 positive 14.54 HSV-1 positive 15.53 HSV-1
F rash/lesion positive HSV-2 positive 14.87 HSV-2 positive 17.99 HSV-2
M rectal positive HSV-2 positive 15.84 HSV-2 positive 20.13 HSV-2
F genital negativec n/a positive 17.91 HSV-2 positive 19.88 HSV-2
M rash/lesion positive HSV-2 positive 18.35 HSV-2 positive 21.31 HSV-2
M rectal positive HSV-2 positive 19.96 HSV-2 positive 21.58 HSV-2
F rash/lesion positive HSV-1 positive 20.73 HSV-1 positive 22.36 HSV-1
M genital positive HSV-2 positive 23.56 HSV-2 positive 24.98 HSV-2
M genital negative n/a negative   negative negative   negative
M rash/lesion negative n/a negative   negative negative   negative
F genital negative n/a negative   negative negative   negative
M rectal negative n/a negative   negative negative   negative
  1. a 5 ul of extracted nucleic acid used in PCR
  2. b 1 ul of straight clinical specimen used in PCR
  3. c specimen was negative for HSV by cell culture; positive by real-time PCR
  4. n/a specimen was not analyzed
  5. CP value Crossing Point of real-time PCR