Real-Time PCR for detection of herpes simplex virus without nucleic acid extraction

Pandori, Mark W; Lei, John; Wong, Ernest H; Klausner, Jeffrey; Liska, Sally

doi:10.1186/1471-2334-6-104

BMC Infectious Diseases

Table 1 Prospective Clinical HSV Specimens

From: Real-Time PCR for detection of herpes simplex virus without nucleic acid extraction

Specimen		Culture/IFA		Real-Time PCR
				Extracted^a			Unextracted^b
Gender	Site	Culture	IFA	Result	CP value	Type	Result	CP value	Type
F	genital	positive	HSV-2	positive	9.87	HSV-2	positive	12.49	HSV-2
M	genital	positive	HSV-2	positive	10.95	HSV-2	positive	12.16	HSV-2
M	genital	positive	HSV-1	positive	11.03	HSV-1	positive	12.18	HSV-1
F	genital	positive	HSV-2	positive	11.82	HSV-2	positive	12.70	HSV-2
F	genital	positive	HSV-2	positive	13.10	HSV-2	positive	15.28	HSV-2
M	genital	positive	HSV-2	positive	13.35	HSV-2	positive	15.69	HSV-2
F	genital	positive	HSV-2	positive	13.80	HSV-2	positive	16.83	HSV-2
F	genital	positive	HSV-2	positive	13.97	HSV-2	positive	15.20	HSV-2
M	genital	positive	HSV-1	positive	15.27	HSV-1	positive	16.09	HSV-1
M	genital	negative^c	n/a	positive	16.06	HSV-1	positive	18.18	HSV-1
F	genital	positive	HSV-2	positive	16.97	HSV-2	positive	17.22	HSV-2
M	genital	positive	HSV-2	positive	17.47	HSV-2	positive	18.85	HSV-2
F	genital	positive	HSV-2	positive	17.60	HSV-2	positive	18.49	HSV-2
M	rectal	positive	HSV-2	positive	19.48	HSV-2	positive	21.82	HSV-2
M	genital	positive	HSV-1	positive	20.37	HSV-1	positive	22.29	HSV-1
M	genital	positive	HSV-2	positive	20.66	HSV-2	positive	22.60	HSV-2
F	genital	positive	HSV-2	positive	22.64	HSV-2	positive	23.92	HSV-2
F	genital	negative	n/a	negative		negative	negative		negative
M	rectal	negative	n/a	negative		negative	negative		negative
M	rectal	negative	n/a	negative		negative	negative		negative
F	rectal	negative	n/a	negative		negative	negative		negative
M	rash/lesion	negative	n/a	negative		negative	negative		negative
M	genital	negative	n/a	negative		negative	negative		negative
F	genital	negative	n/a	negative		negative	negative		negative
M	rectal	negative	n/a	negative		negative	negative		negative
M	genital	negative	n/a	negative		negative	negative		negative
M	genital	negative	n/a	negative		negative	negative		negative
F	genital	negative	n/a	negative		negative	negative		negative
M	rectal	negative	n/a	negative		negative	negative		negative
M	genital	negative	n/a	negative		negative	negative		negative
M	genital	negative	n/a	negative		negative	negative		negative
M	rash/lesion	negative	n/a	negative		negative	negative		negative
M	rectal	negative	n/a	negative		negative	negative		negative

^a 5 ul of extracted nucleic acid used in PCR
^b 1 ul of straight clinical specimen used in PCR
^c specimen was negative for HSV by cell culture; positive by real-time PCR
n/a specimen was not analyzed
CP value Crossing Point of real-time PCR

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ISSN: 1471-2334

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