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Table 1 Prospective Clinical HSV Specimens

From: Real-Time PCR for detection of herpes simplex virus without nucleic acid extraction

Specimen Culture/IFA Real-Time PCR
     Extracteda Unextractedb
Gender Site Culture IFA Result CP value Type Result CP value Type
F genital positive HSV-2 positive 9.87 HSV-2 positive 12.49 HSV-2
M genital positive HSV-2 positive 10.95 HSV-2 positive 12.16 HSV-2
M genital positive HSV-1 positive 11.03 HSV-1 positive 12.18 HSV-1
F genital positive HSV-2 positive 11.82 HSV-2 positive 12.70 HSV-2
F genital positive HSV-2 positive 13.10 HSV-2 positive 15.28 HSV-2
M genital positive HSV-2 positive 13.35 HSV-2 positive 15.69 HSV-2
F genital positive HSV-2 positive 13.80 HSV-2 positive 16.83 HSV-2
F genital positive HSV-2 positive 13.97 HSV-2 positive 15.20 HSV-2
M genital positive HSV-1 positive 15.27 HSV-1 positive 16.09 HSV-1
M genital negativec n/a positive 16.06 HSV-1 positive 18.18 HSV-1
F genital positive HSV-2 positive 16.97 HSV-2 positive 17.22 HSV-2
M genital positive HSV-2 positive 17.47 HSV-2 positive 18.85 HSV-2
F genital positive HSV-2 positive 17.60 HSV-2 positive 18.49 HSV-2
M rectal positive HSV-2 positive 19.48 HSV-2 positive 21.82 HSV-2
M genital positive HSV-1 positive 20.37 HSV-1 positive 22.29 HSV-1
M genital positive HSV-2 positive 20.66 HSV-2 positive 22.60 HSV-2
F genital positive HSV-2 positive 22.64 HSV-2 positive 23.92 HSV-2
F genital negative n/a negative   negative negative   negative
M rectal negative n/a negative   negative negative   negative
M rectal negative n/a negative   negative negative   negative
F rectal negative n/a negative   negative negative   negative
M rash/lesion negative n/a negative   negative negative   negative
M genital negative n/a negative   negative negative   negative
F genital negative n/a negative   negative negative   negative
M rectal negative n/a negative   negative negative   negative
M genital negative n/a negative   negative negative   negative
M genital negative n/a negative   negative negative   negative
F genital negative n/a negative   negative negative   negative
M rectal negative n/a negative   negative negative   negative
M genital negative n/a negative   negative negative   negative
M genital negative n/a negative   negative negative   negative
M rash/lesion negative n/a negative   negative negative   negative
M rectal negative n/a negative   negative negative   negative
  1. a 5 ul of extracted nucleic acid used in PCR
  2. b 1 ul of straight clinical specimen used in PCR
  3. c specimen was negative for HSV by cell culture; positive by real-time PCR
  4. n/a specimen was not analyzed
  5. CP value Crossing Point of real-time PCR