Figure 4
Cell proliferation (U-937 Human macrophage cell line) was determined by XTT assay to evaluate the cytotoxic effect of different venoms (a – e) and PLA2s (f – j) exposed at different time intervals. (a) South American rattlesnake (Crotalus adamanteus), (b) Russells viper (Daboia russelli russelli), (c) King brown (Pseudechis australis), (d) Pallas (Agkistrodon halys) (e) Speckled brown (Pseudechis guttata) (f) Crotoxin B (Crotalus durissus terrificus), (g) Daboiatoxin (Daboia russelli russelli), (h) Melittin (Apis mellifera), (i) Mulgatoxin (Pseudechis australis) (j) Crotoxin A (Crotalus durissus terrificus). Macrophages were incubated with varying concentrations of venoms (0.05 – 10 mg/ml) and PLA2s (0.05 – 10 μg/ml).