DENV-Ag and iNOS expression after
Dengue infection of monocytes. Adherent human PBMLs were incubated for three days with infected DENV (Figures 4A, 4B and 4C). Cells were labelled either with isotype matched antibodies (Figures 6A and 6B) or antibodies to CD14-PE (y-axis) and DENV-Complex-Alexa-654 (x-axis) and were analysed by FACS (Figure 4C, ungated). Cells incubated for three to six days with cell culture medium, inactivated DENV or infectious DENV were labelled with antibody to DENV-Ag and anti-mouse IgG-PE and/or anti-iNOS-FITC and analysed by FACS. Isotype matched antibodies were used for both labelings (Figure 4D, 3 days for DENV-Ag and 6 days for iNOS labelling). x-axis represent mean of fluorescence intensity and y-axis represent percentage of maximum cell number counts. Figure 4E shows a kinetic curve comparing DENV-Ag and iNOS expression on cells from the monocyte gate. Each point represent average ± SEM percentages from samples set in triplicates. * P < 0.05 and in Student's T-test showing difference in DENV-Ag and iNOS expression compared to uninfected controls (CT). Data are obtained from one representative experiment out of three performed with different cell donors.