Dot and contour plots showing DENV-Ag and iNOS monocyte and lymphocyte expression in dengue patients. Human PBMLs a from healthy individual (Figures 1A,1D,1G) were used as control. Cells from a 4-day dengue infected patient (Figures 1B,1C,1E,1F,1H,1I) were labelled with anti-CD14-PE and CD14+ gated cells – R1 (Figure 1B) were considered as logical gate or monocyte gate and R2 is as the lymphocyte gate in the FCS vs. SSC dot plot (Figure 1C). R1 and R2 were used for further analysis during this work. Alternatively, human PBMLs were labelled either with an antibody to DENV Ags and anti-mouse IgG-PE (Figures 1D-1F) or with anti-iNOS-FITC (Figures 1G-1I) and analysed by FACS in either the R1, monocyte gate (Figures 1D-1F) or in the R2, lymphocyte gate (Figures 1F-1I). x-axis represent mean of cell population size (FCS) and y-axis represent cell granularity (SSC) or fluorescence intensity.