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Figure 4 | BMC Infectious Diseases

Figure 4

From: Rapid and simultaneous detection of human hepatitis B virus and hepatitis C virus antibodies based on a protein chip assay using nano-gold immunological amplification and silver staining method

Figure 4

The effects of various silver enhancement time on signal intensity. The concentration of antigens spotted on the slides was 100 μg/ml. The silver enhancement time varied from 5 to 15 minutes. The result indicated the optimum time for silver development ranged from 8 to 12 minutes. Both too short (less than 5 minutes) and too long (more than 15 minutes) development might bring on darkened background.

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