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Table 1 PCR efficiencies of HIV-1 group M amplification

From: Human immunodeficiency virus type 1 (HIV-1) proviral DNA load in purified CD4+ cells by LightCycler® Real-time PCR

Sample concentration:

Subtype A

Subtype B

Subtype C

Subtype D

Log dilution

Mean Cpa

Mean Cp

Mean Cp

Mean Cp

-2

26,16

22,90

24,61

26,13

-3

29,50

26,02

28,10

29,27

-4

32,82

29,75

31,79

32,86

-5

36,72

32,98

34,83

36,72

Slopeb

-3,50

-3,37

-3,43

-3,54

R2c

0,99

0,99

0,99

0,99

  1. (a) Mean of crossing point values calculated using two replicates of each point in 3 runs.
  2. (b) The LightCycler Software calculates the slope of the curve by plotting crossing points against the logarithm of concentration for each point of a 10-fold dilution series. A slope value of -3.33 indicates the maximal PCR efficiency.
  3. (c) The LightCycler Software also calculates the coefficient of correlation R2.