Figure 2

Validation of the use of 8E5 cells as standards for human globin PCR. The figure shows similar results of the 8E5 cell count as performed by the Coulter automated hematology analyzer compared with the values obtained by real-time PCR quantification of the human globin gene. The comparison of the theoretical and calculated values of the DNA control sample from LightCycler Kit shows equal results. Each standard point was run in five replicates.