Table 1 An overview of the primers and probes used in the dual fluorescent multiprobe assay
From: A dual fluorescent multiprobe assay for prion protein genotyping in sheep
Primer/Probe Name | Sequence (5' → 3') | Length (bp) | Tm (°C) | avTm (°C) |
|---|---|---|---|---|
Forward primer | GCCTTGGTGGCTACATG | 17 | 59.35 | - |
Reverse primer | CTGTGATGTTGACACAGTCAT | 21 | 59.60 | - |
A136-probe | FAM-TGCTCATGGCACTTCCCA-BHQ1 | 18 | 62.98 | 56.27 |
V136-probe | HEX-CTGCTCATGACACTTCCCAG-BHQ1 | 20 | 61.86 | 55.33 |
R154-probe | TexasRed-CCGTTACTATCGTGAAAACATGTAC-BHQ2 | 25 | 61.08 | 56.59 |
H154-probe | Cy5-CCGTTACTATCATGAAAACATGTACC-BHQ2 | 26 | 61.06 | 56.67 |
R171-probe | FAM-CCAGTGGATCGGTATAGTAACCA-BHQ1 | 23 | 62.27 | 57.19 |
H171-probe | HEX-AGACCAGTGGATCATTATAGTAACCA-BHQ1 | 26 | 61.96 | 57.66 |
Q171-probe | TexasRed-CCAGTGGATCAGTATAGTAACCAGA-BHQ2 | 25 | 62.07 | 58.46 |