(A-G) – Amplification plots obtained with the dual fluorescent multiprobe assay for sheep
genotyping. Amplification plots are shown for a homozygous positive (+/+), a heterozygote (+/-), a homozygous negative (-/-) and a no template control (NTC) with (A) A136-probe (FAM-labelled) in PCR 1, (B) V136-probe (HEX-labelled) in PCR 1, (C) R154-probe (TexasRed-labelled) in PCR 1, (D) H154-probe (Cy5-labelled) in PCR 1, (E) R171-probe (FAM-labelled) in PCR 2, (F) H171-probe (HEX-labelled) in PCR 2 and (G) Q171-probe (TexasRed-labelled) in PCR 2. (H) An amplification plot for 41 test samples and 9 control samples for the latter probe. For all amplification plots, unpurified DNA was used as a template.