From: Comparison of nested PCR and real time PCR of Herpesvirus infections of central nervous system in HIV patients
NESTED PCR
REAL TIME PCR
POS
NEG
CMV
85
150
HSV-1
45
150+1a = 151
45+1b = 46
149+1a = 150
EBV
145
145 +1b = 146
149