Identification of two
strains by DGGE using PCR amplified segments of the 16S rRNA gene Panel A, lane 1 is from vaginal lavage sample #7189A obtained from a patient with BV. Note the closely opposed A. vaginae bands in the boxed area. Lanes 2 and 3 show the individual A. vaginae bands from the same sample after extraction and re-amplification of the two bands in lane 1. Panel B, lane 1 is from a vaginal lavage sample #7200A obtained from another BV positive patient #7200. It also shows the double A. vaginae band pattern (boxed area). Lane 2 shows the band obtained from an A. vaginae strain isolated from patient #7200. This band co-migrates with the lower of the two bands obtained from this patient's vaginal lavage sample. Lane 3 shows the band obtained from the ATCC A. vaginae culture collection strain. It co-migrates with the upper of the two A. vaginae bands shown in lane 1. The slight difference in the electrophoretic migration of the A. vaginae bands correlates with a single nucleotide difference in their amplicons.