Vaccine candidates derived from a novel infectious cDNA clone of an American genotype dengue virus type 2

BMC Infectious Diseases

Table 5 Addition of the 4995 mutation further attenuates rDEN2Δ30 in SCID-HuH-7 mice.

	Replication in SCID-HuH-7 mice^a
Virus	No. of mice	Mean virus titer (log₁₀PFU/ml ± SE)
DEN-2 Tonga/74	6	5.9 ± 0.3
rDEN-2	15	6.4 ± 0.2
rDEN2Δ30	22	5.2 ± 0.1
rDEN2Δ30-4995	28	4.6 ± 0.2

^a Groups of HuH-7-SCID mice were inoculated directly into the tumor with 10⁴ PFU virus. Serum was collected on day 7 and titered in Vero cells. Data is included from three separate experiments.

ISSN: 1471-2334