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Table 3 Calculation of avidity indices from dilution series of representative samples with the software "Avidity 1.2" and with the new calculation method described in this paper.

From: A new method for determination of varicella-zoster virus immunoglobulin G avidity in serum and cerebrospinal fluid

(a) Raw data of dilution series

 

Serum F19.3

Serum P28

Serum V9.2

Relative dilution1

Abs (ref)2

Abs (denat)3

Abs (ref)

Abs (denat)

Abs (ref)

Abs (denat)

1:1

2.765

0.925

2.668

1.600

2.936

1.948

1:2

1.962

0.626

1.833

1.144

1.943

1.268

1:4

1.428

0.356

1.300

0.727

1.269

0.782

1:8

0.831

0.190

0.824

0.441

0.778

0.463

1:16

0.592

0.106

0.480

0.252

0.409

0.192

(b) Avidity indices obtained from different combinations of dilutions with arithmetic mean, standard deviation (SD) and coefficient of variation (CV)

 

Serum F19.3

Serum P28

Serum V9.2

Dilutions used for calculation

Avidity 1.2 (%)

new method (%)

Avidity 1.2 (%)

new method (%)

Avidity 1.2 (%)

new method (%)

1:1 & 1:2

9.5

32.0

33.4

61.8

46.1

65.4

1:2 & 1:4

15.2

32.3

40.4

63.7

49.2

65.5

1:4 & 1:8

18.0

25.8

41.5

57.4

50.0

62.9

1:8 & 1:16

11.7

30.0

44.6

54.4

56.6

68.0

1:1 & 1:4

11.6

30.0

35.5

58.7

48.2

64.7

1:2 & 1:8

17.3

32.0

41.8

63.0

50.1

65.4

1:4 & 1:16

13.5

26.4

43.5

56.6

50.2

64.2

1:1 & 1:8

14.7

31.7

41.3

59.0

52.8

65.2

1:2 & 1:16

15.0

32.0

42.0

62.7

51.3

65.4

Mean

14.1

30.2

40.4

59.7

50.5

65.2

SD

2.8

2.5

3.7

3.3

3.0

1.4

CV

19.7

8.4

9.0

5.5

5.9

2.1

  1. 1Relative dilution: the starting dilutions were 1:1512 (F19.3), 1:144 (P28), 1:399168 (V9.2)
  2. 2absorbance value in EIA without denaturing washing step
  3. 3absorbance value in EIA with denaturing washing step