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Table 3 Calculation of avidity indices from dilution series of representative samples with the software "Avidity 1.2" and with the new calculation method described in this paper.

From: A new method for determination of varicella-zoster virus immunoglobulin G avidity in serum and cerebrospinal fluid

(a) Raw data of dilution series
  Serum F19.3 Serum P28 Serum V9.2
Relative dilution1 Abs (ref)2 Abs (denat)3 Abs (ref) Abs (denat) Abs (ref) Abs (denat)
1:1 2.765 0.925 2.668 1.600 2.936 1.948
1:2 1.962 0.626 1.833 1.144 1.943 1.268
1:4 1.428 0.356 1.300 0.727 1.269 0.782
1:8 0.831 0.190 0.824 0.441 0.778 0.463
1:16 0.592 0.106 0.480 0.252 0.409 0.192
(b) Avidity indices obtained from different combinations of dilutions with arithmetic mean, standard deviation (SD) and coefficient of variation (CV)
  Serum F19.3 Serum P28 Serum V9.2
Dilutions used for calculation Avidity 1.2 (%) new method (%) Avidity 1.2 (%) new method (%) Avidity 1.2 (%) new method (%)
1:1 & 1:2 9.5 32.0 33.4 61.8 46.1 65.4
1:2 & 1:4 15.2 32.3 40.4 63.7 49.2 65.5
1:4 & 1:8 18.0 25.8 41.5 57.4 50.0 62.9
1:8 & 1:16 11.7 30.0 44.6 54.4 56.6 68.0
1:1 & 1:4 11.6 30.0 35.5 58.7 48.2 64.7
1:2 & 1:8 17.3 32.0 41.8 63.0 50.1 65.4
1:4 & 1:16 13.5 26.4 43.5 56.6 50.2 64.2
1:1 & 1:8 14.7 31.7 41.3 59.0 52.8 65.2
1:2 & 1:16 15.0 32.0 42.0 62.7 51.3 65.4
Mean 14.1 30.2 40.4 59.7 50.5 65.2
SD 2.8 2.5 3.7 3.3 3.0 1.4
CV 19.7 8.4 9.0 5.5 5.9 2.1
  1. 1Relative dilution: the starting dilutions were 1:1512 (F19.3), 1:144 (P28), 1:399168 (V9.2)
  2. 2absorbance value in EIA without denaturing washing step
  3. 3absorbance value in EIA with denaturing washing step