Table 2 Quasispecies fluctuations and mutations during the transition from human tissue to Vero cell culture. Nucleotide variations observed between primary human tissue isolates and their respective subsequent Vero cell culture. Both quasispecies selection (SIN3275V → SIN849M) and new emergence (AS → HSR1) are observed during the transmission of SARS-CoV from human tissue sample into Vero cell culture.
From: Mutational dynamics of the SARS coronavirus in cell culture and human populations isolated in 2003
Source of viral sequence: Human Tissue | Source of viral sequence: Passage to Vero cell culture | Nucleotide Heterogeneity | Position (Urbani) | ORF (based on NC_004718.3) |
|---|---|---|---|---|
Sin3408L | Sin842M | - | - | - |
Sin3725V | Sin849M | Y [t/C] → T | 548 | 95aa of Leader protein (I, T → I) |
|  |  | Y [T/C] → C | 13347 | 131aa of NSP10 (SILENT) |
Sin3765V | Sin852M | - | - | - |
AS | HSR 1 | G → R [G/A] | 27254 | 637aa of sars6 (D → D, N) |