A robust, low- to medium-throughput prnpgenotyping system in sheep

BMC Infectious Diseases

Table 1 ARMS primers used to type the ovine PRNP

Name	Sequence of Primer 5'-3'	Allele	Length/Label	Fragment Length
PRNP_136A	ATACATGCTGGGAAGTGC	A¹³⁶	18 bp/TET	-
PRNP_136V	CTACATGCTGGGAAGTGT	V¹³⁶	18 bp/FAM	-
PRNP_154R	ACTAACGGTACATGTTTTCAC	R¹⁵⁴	21 bp/-	92 bp
PRNP_154H	AGTTTGTAACGGTACATGTTTTgAT	H¹⁵⁴	25 bp/-	96 bp
PRNP_171Q	GGAAGTTGTTCTGGTTACTATcCT	Q¹⁷¹	24 bp/-	146 bp
PRNP_171R	TCAGTTAAGTTGTTCTGGTTACTATtCC	R¹⁷¹	28 bp/-	150 bp
PRNP_171H	TTCTGAGCATAAAGTTGTTCTGGTTACTATAA	H¹⁷¹	32 bp/-	154 bp

Small letters indicate deliberately introduced mismatches (ARMS principle); underlining indicate pieces of neutral sequence that were added to elongate the primer to allow allele-discrimination by capillary electrophoresis.

ISSN: 1471-2334