Ultraviolet-treated EBs colocalize with caveolin-2 in HeLa cells. Chlamydial EBs were treated with UV light for 1 h and then used to infect HeLa 229 HeLa cells for 36 h. The cells were fixed and stained with a mouse anti-caveolin-2 and a guinea pig anti-Chlamydia antibody. A FITC-conjugated goat anti-mouse antibody and a TRITC-conjugated goat anti-guinea pig secondary antibody were used to visualize the stained EBs. Unlike the case in FRT cells, UV-treated EBs colocalized with caveolin-2 in HeLa cells, which express both Caveolin-1 and Caveolin-2. The above confocal micrographs are representative of the morphology of all Chlamydiaceae that colocalize with caveolin-2 (C. pneumoniae, GPIC, and C. trachomatis serovars A, B, C, E, F and K). Original magnification: 600 X. Each scale bar represents 15 μm.