Figure 5

Ultraviolet-treated EBs do not colocalize with caveolin-2 in caveolin-1 negative FRT cells. Chlamydial EBs were treated with short wavelength UV for 1 h and then used to infect FRT HeLa cells for 36 h. The cells were fixed and stained with a mouse anti-caveolin-2 and a guinea pig anti-Chlamydia antibody. FITC-conjugated goat anti-mouse as well as TRITC-conjugated goat anti-guinea pig secondary antibody was used to visualize the stained EBs. The representative confocal micrographs above show that UV-treated EBs did not colocalize with caveolin-2 in FRT cells. All of the Chlamydiaceae not shown had a similar morphology. Scale bar represents 15 μm Original magnification: 600X