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Figure 4 | BMC Infectious Diseases

Figure 4

From: I-TRAP: A method to identify transcriptional regulator activated promoters

Figure 4

Map of pSE.4. pSE.4 is a promoter-trap plasmid containing a promoterless aph gene and xylE gene, an E. coli rRNA transcriptional terminator cloned upstream of the promoterless aph and xylE genes, a unique BamHI cloning site, an oriM for plasmid replication in mycobacteria, an oriE for plasmid replication in E. coli, and a hyg gene for selection in E. coli and M. smegmatis bacteria. Arrowheads indicate direction of transcription.

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