Figure 3
From: I-TRAP: A method to identify transcriptional regulator activated promoters
Map of pSEKO.4 pSEKO.4 contains the M. smegmatis sigE gene interrupted with a gentamicin-resistance gene, aac1, the lacZ gene for use as a screen against single recombination events, and an oriE for plasmid replication in E. coli. Arrowheads indicate direction of transcription. The construction of the mutated sigE gene resulted in an insertion of the aac1 cassette and a 2-bp deletion of the sigE gene at the site of insertion.