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Figure 2 | BMC Infectious Diseases

Figure 2

From: I-TRAP: A method to identify transcriptional regulator activated promoters

Figure 2

Map of pSEint.2. pSEint.2 contains an oriE for plasmid replication in E. coli, the mycobacteriophage L5 integrase (int) and attP to allow integration into the M. smegmatis chromosome, a ble gene (bleomycin resistance) for selection in mycobacteria, an amp gene (ampicillin resistance) for selection in E. coli, and the M. tuberculosis sigE gene under the control of the constitutively expressed hsp60 promoter. Arrowheads indicate direction of transcription.

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