Figure 8

Inactivation of HIV-1 IIIB by polymeric anionic candidate microbicides. HIV-1 IIIB (7 × 10⁹ virus particles/100 μl) was treated with the compounds indicated on the abscissa (final concentrations 10 mg/ml except for Carbomer 974P [2.5 mg/ml; neutralized to pH 7.0; at higher concentrations the solutions became too viscous to handle]) for 5 min at 37°C. The final dilution of BufferGel was 4-fold (final pH = 4.0). The preparations were cooled on ice and virus particles were separated from the polymers and their infectivity determined as described in the Methods section. For comparison, results obtained with a formulation of micronized CAP (= Aquateric; see legend for Fig. 2) are shown.