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Figure 4 | BMC Infectious Diseases

Figure 4

From: Anti-HIV-1 activity of anionic polymers: a comparative study of candidate microbicides

Figure 4

gp41 six-helix bundle induction in HIV-1 IIIB by distinct anionic polymers representing candidate microbicides. Purified HIV-1 IIIB (7 × 109 particles in TS) was precipitated with 3% PEG. The pellets were resuspended in 100 μl of phosphate buffer saline (PBS) to separate the virus from soluble HIV-1 antigens in the preparation. The different anionic polymers (listed on the ordinate) were added to a final concentration of 5 mg/ml except for Carbomer 974P (2.5 mg/ml). After incubation for 5 min at 37°C, the mixtures were cooled in ice and PEG was added (final concentration 3%). The pellets after centrifugation were washed once with 3% PEG in PBS, repelletted and finally resuspended in PBS containing 1% NP40 and 100 μg/ml BSA and incubated at 20°C for 30 min. The formation of six-helix bundles was determined by ELISA (see Methods section). All experiments were done in triplicate, the absorbance readings are shown as percentages of the absorbance corresponding to six-helix bundles elicited by CAP (= 100%).

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