Skip to main content
Figure 1 | BMC Infectious Diseases

Figure 1

From: Anti-HIV-1 activity of anionic polymers: a comparative study of candidate microbicides

Figure 1

Binding of HIV-1 IIIB gp120 to micronized CAP and Aquateric, respectively. Graded quantities of micronized CAP and Aquateric, respectively, in 0.5 ml of 0.14 M NaCl were mixed with 100 μl of a solution containing 1 μg of HIV-1 IIIB gp120, and 10 μg each of bovine serum albumin (BSA) and gelatin. After incubation for 5 min at 37°C the mixtures were cooled on ice, centrifuged at 10,000 rpm and the supernatant fluids were filtered through 0.45 μ filters which had been prewashed with a solution containing 100 μg/ml of each BSA and gelatin in 0.14 M NaCl. gp120 in the filtrates was determined as described in the Methods section. The percentage of gp120 recovered in the filtrates was determined from calibration curves relating absorbance readings to the concentration of gp120.

Back to article page