Figure 3

Detection of PrP ^Sc in TSEs from different host species. Lanes show: 1 – mouse scrapie ME7, 2 – sheep scrapie, 3 – mouse BSE, 4 – BSE, 5 – sporadic CJD, 6 – normal sheep. Lanes 1 and 3 contain 2 mg and lanes 2, 4, 6 contain 3 mg brain equivalent load each. SAL 1 (1:3000) and alkaline phosphatase labeled goat anti-rabbit IgG (1:1500, Roche) were the primary and the secondary antibodies respectively. Amounts of proteinase K used per ml homogenate were 30 μg in the cases of mouse scrapie, sheep scrapie and BSE and 50 μg in the case of sCJD.