From: Stable hepatitis C virus RNA detection by RT-PCR during four days storage
Tube 1 without separator => | centrifuge within 1 hour => | 1 aliquot (time 0) at -80°C => | tube kept at 4°C => | aliquots at -80°C | at 6 hours at 24 hours at 48 hours at 72 hours at 96 hours |
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Tube 2 without separator => | centrifuge within 1 hour => | tube kept at room t° => | aliquots at -80°C | at 6 hours at 24 hours at 48 hours at 72 hours at 96 hours | |
Tube 3 with separator => | centrifuge within 1 hour => | make 6 aliquots and keep one (time 0) at -80°C => | aliquots kept at 4°C => | aliquots at -80°C | at 6 hours at 24 hours at 48 hours at 72 hours at 96 hours |
Tube 4 with separator => | centrifuge within 1 hour => | make 5 aliquots => | aliquots at room t° => | aliquots at -80°C | at 6 hours at 24 hours at 48 hours at 72 hours at 96 hours |
Tube 5 with separator => | at room t° for 6 hours then centrifuged => | make 9 aliquots and keep 1 aliquot (6 h) at -80°C => | 4 aliquots at 4°C => | aliquots at -80°C | at 24 hours at 48 hours at 72 hours at 96 hours |
4 aliquots at room t° | aliquots at -80°C | at 24 hours at 48 hours at 72 hours at 96 hours |