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Table 1 Serum tube handling before testing

From: Stable hepatitis C virus RNA detection by RT-PCR during four days storage

Tube 1 without

separator =>

centrifuge within

1 hour =>

1 aliquot (time 0) at -80°C =>

tube kept at 4°C =>

aliquots at -80°C

at 6 hours

at 24 hours

at 48 hours

at 72 hours

at 96 hours

Tube 2 without

separator =>

centrifuge within

1 hour =>

 

tube kept at room t° =>

aliquots at -80°C

at 6 hours

at 24 hours

at 48 hours

at 72 hours

at 96 hours

Tube 3 with

separator =>

centrifuge within

1 hour =>

make 6 aliquots and keep one (time 0)

at -80°C =>

aliquots kept at 4°C =>

aliquots at -80°C

at 6 hours

at 24 hours

at 48 hours

at 72 hours

at 96 hours

Tube 4 with

separator =>

centrifuge within

1 hour =>

make 5 aliquots =>

aliquots at room t° =>

aliquots at -80°C

at 6 hours

at 24 hours

at 48 hours

at 72 hours

at 96 hours

Tube 5 with

separator =>

at room t° for 6 hours

then centrifuged =>

make 9 aliquots and

keep 1 aliquot (6 h) at -80°C =>

4 aliquots at 4°C =>

aliquots at -80°C

at 24 hours

at 48 hours

at 72 hours

at 96 hours

   

4 aliquots at room t°

aliquots at -80°C

at 24 hours

at 48 hours

at 72 hours

at 96 hours