Table 1 Serum tube handling before testing
From: Stable hepatitis C virus RNA detection by RT-PCR during four days storage
|
Tube 1 without separator => |
centrifuge within 1 hour => | 1 aliquot (time 0) at -80°C => | tube kept at 4°C => | aliquots at -80°C |
at 6 hours at 24 hours at 48 hours at 72 hours at 96 hours |
|---|---|---|---|---|---|
|
Tube 2 without separator => |
centrifuge within 1 hour => | tube kept at room t° => | aliquots at -80°C |
at 6 hours at 24 hours at 48 hours at 72 hours at 96 hours | |
|
Tube 3 with separator => |
centrifuge within 1 hour => |
make 6 aliquots and keep one (time 0) at -80°C => | aliquots kept at 4°C => | aliquots at -80°C |
at 6 hours at 24 hours at 48 hours at 72 hours at 96 hours |
|
Tube 4 with separator => |
centrifuge within 1 hour => | make 5 aliquots => | aliquots at room t° => | aliquots at -80°C |
at 6 hours at 24 hours at 48 hours at 72 hours at 96 hours |
|
Tube 5 with separator => |
at room t° for 6 hours then centrifuged => |
make 9 aliquots and keep 1 aliquot (6 h) at -80°C => | 4 aliquots at 4°C => | aliquots at -80°C |
at 24 hours at 48 hours at 72 hours at 96 hours |
| 4 aliquots at room t° | aliquots at -80°C |
at 24 hours at 48 hours at 72 hours at 96 hours |