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Table 1 Serum tube handling before testing

From: Stable hepatitis C virus RNA detection by RT-PCR during four days storage

Tube 1 without
separator =>
centrifuge within
1 hour =>
1 aliquot (time 0) at -80°C => tube kept at 4°C => aliquots at -80°C at 6 hours
at 24 hours
at 48 hours
at 72 hours
at 96 hours
Tube 2 without
separator =>
centrifuge within
1 hour =>
  tube kept at room t° => aliquots at -80°C at 6 hours
at 24 hours
at 48 hours
at 72 hours
at 96 hours
Tube 3 with
separator =>
centrifuge within
1 hour =>
make 6 aliquots and keep one (time 0)
at -80°C =>
aliquots kept at 4°C => aliquots at -80°C at 6 hours
at 24 hours
at 48 hours
at 72 hours
at 96 hours
Tube 4 with
separator =>
centrifuge within
1 hour =>
make 5 aliquots => aliquots at room t° => aliquots at -80°C at 6 hours
at 24 hours
at 48 hours
at 72 hours
at 96 hours
Tube 5 with
separator =>
at room t° for 6 hours
then centrifuged =>
make 9 aliquots and
keep 1 aliquot (6 h) at -80°C =>
4 aliquots at 4°C => aliquots at -80°C at 24 hours
at 48 hours
at 72 hours
at 96 hours
    4 aliquots at room t° aliquots at -80°C at 24 hours
at 48 hours
at 72 hours
at 96 hours