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Table 1 Studies with controls of Chlamydia pneumoniae DNA detection in peripheral blood mononuclear cells

From: Association of circulating Chlamydia pneumoniaeDNA with cardiovascular disease: a systematic review

Reference

Country

Cases

Prevalence (%)

Controls

Prevalence (%)

Blood sampling

DNA Extraction

PCRa target

Berger, 2000 [21]

Germany

Vascular surgery

12 of 60 (20.0)

Not stated

7 of 51 (13.7)

4 mL EDTA blood

Phenol-chloroform

16S rDNA

Boman, 1998 [22]

Sweden

Coronary Angiography

60 of 101 (59.4)

Blood donors

24 of 52 (46.1)

10 mL EDTAb blood

Phenol-chloroform

Nested MOMPc

Da Costa, 2000 (abstract)

Germany

Coronary Angioplasty and antibiotics

14 of 233 (6.0)

Blood donors

5 of 200 (2.5)

Not stated

QIAamp or phenol-chloroform

Not stated

Freidank, 2000 [23]

Germany

Carotid atherosclerosis

10 of 60 (16.7)

Medical students, patients

0 of 109 (0.0)

10 mL whole blood

QIAamp

Nested pstI cloned fragment

Kaul, 2000 [24]

United States

Coronary artery disease

13 of 28 (46.4)

Blood donors

5 of 19 (26.3)

20–30 mL EDTA, CD3+ cell selection

SDS-proteinase K

Nested MOMP HSPd-60

Maraha, 2001 [25]

Netherlands

Aortic aneurysm

18 of 88 (20.5)

Healthy controls

8 of 88 (9.1)

EDTA blood

QIAamp

16S rDNA

Sessa, 2000 [26]

Italy

Unstable angina, myocardial infarction

24 of 93 (25.8)

Healthy controls

2 of 42 (4.8)

EDTA blood

Phenol-chloroform

Hemi-nested pstI cloned fragment

Smieja, 2001 [27]

Canada

Coronary Angiography or angioplasty

22 of 187 (11.8)

Normal angiogram

2 of 21 (9.5)

8 mL BD CPTc tube

QIAamp

Nested MOMP

Wong, 1999 [28]

United Kingdom

Coronary Angiography

79 of 913 (8.7)

Normal angiogram

21 of 292 (7.2)

4–5 mL EDTA blood

Phenol-chloroform

Nested MOMP

  1. a PCR, polymerase chain reaction b EDTA, ethylene diamine tetra-acetic acid c MOMP, major outer membrane protein d HSP, heat shock protein e CPT, BD cell preparation tube (BD Vacutainer Systems, Franklin Lakes NJ, USA)