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Table 1 Cycling protocol for HBD-1, HBD-2 and GAPDH RT-PCR

From: Expression of human beta-defensins 1 and 2 in kidneys with chronic bacterial infection

Step Denaturation time/temperature Annealing time/temperature Extension time/temperature Number of cycles
HBD-1     
1 1 5 min/95°C 30 sec/55°C 30 sec/72°C 1
2 30 sec/95°C 30 sec/55°C 30 sec/72°C 28
3 30 sec/95°C 30 sec 55°C 10 min/72°C 1
HBD-2     
1 1 5 min/95°C 30 sec/60°C 30 sec/72°C 1
2 30 sec/95°C 30 sec/60°C 30 sec/72°C 28
3 30 sec/95°C 30 sec/60°C 10 min/72°C 1
2 GAPDH     
1 5 min/95°C 30 sec/55°C or 60°C 30 sec/72°C 1
2 30 sec/95°C 30 sec/55°C or 60°C 30 sec/72°C 28
3 30 sec/95°C 30 sec/55°C or 60°C 10 min/72°C 1
  1. 1 Taq Polymerase added at the end of the first 5 min denaturation step. 2GAPDH RT-PCR was performed as duplex PCR in concert with either HBD-1 or HBD-2