• NS5B interacts with different host factors for viral replication [14–20] and virus morphogenesis .|
• NS5B represents a therapeutic target for the inhibition of viral replication [27, 35, 44].
• NS5B is responsible for high variability of HCV .
• NS5B viral genomic region is necessary for phylogenetic and phylodinamic analysis [7–9], but not enough to identify recombinant strains [31, 32, 42].
|Major research questions|
• Is NS5B variability associated with prognosis of the disease in HCV infected patients? |
• Is viral replication fitness influenced in HCV infected and HIV/HCV co-infected patients by NS5B variability? 
• Can adaptive evolution of NS5B contribute to persistence of HCV in PBMCs?