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Table 3 Performance of multiplex PCR in comparison to tissue culture for 130 clinical specimens

From: Detection of a novel avian influenza A (H7N9) virus in humans by multiplex one-step real-time RT-PCR assay

Virus Culture Multiplex WHO Sens. M/W Spec. M/W
+ - + - + -
FluA 13a 117 15 115 15 115 100/100 98.3/98.3
H7 4 126 4 126 4 126 100/100 100/100
N9 4 126 4 126 4 126 100/100 100/100
  1. Sens., sensitivity; Spec., specificity; M/W, Multiplex/WHO. Values refer individually to the virus culture.
  2. aTwo negative samples by tissue culture consisted of one H1N1 (2009) and one H3N2 virus by genomic sequence analysis. Indeterminate results were excluded from sensitivity and specificity calculations.