Detection of a novel avian influenza A (H7N9) virus in humans by multiplex one-step real-time RT-PCR assay

BMC Infectious Diseases

Table 3 Performance of multiplex PCR in comparison to tissue culture for 130 clinical specimens

Virus	Culture		Multiplex		WHO		Sens. M/W	Spec. M/W
Virus	+	-	+	-	+	-	Sens. M/W	Spec. M/W
FluA	13^a	117	15	115	15	115	100/100	98.3/98.3
H7	4	126	4	126	4	126	100/100	100/100
N9	4	126	4	126	4	126	100/100	100/100

Sens., sensitivity; Spec., specificity; M/W, Multiplex/WHO. Values refer individually to the virus culture.
^aTwo negative samples by tissue culture consisted of one H1N1 (2009) and one H3N2 virus by genomic sequence analysis. Indeterminate results were excluded from sensitivity and specificity calculations.

ISSN: 1471-2334