Table 1 Primers and probes designed and used in this study
Set | Primers and probes | Sequences (5′ → 3′) | Targeted genes | Location (bp) | GenBank accession no. |
|---|---|---|---|---|---|
Designed and used in this study | |||||
FluA | FluA forward | GGARTGGMTAAAGACAAGACCAATC | 129-153 | ||
FluA reverse | GGCRTTYTGGACAAASCGTCTAC | Matrix protein | 227-249 | KC885959.1 | |
FluA probe | ROX- AGTCCTCGCTCACTGGGCACGGT-BHQ2 | 199-221 | |||
H7 | H7 forward | GAGGCRATGCAAAATAGAATACAGAT | 1510-1535 | ||
H7 reverse | CCGAAGCTAAACCARAGTATCACA | Hemagglutinin | 1569-1592 | KC885956.1 | |
H7 probe | FAM- ACCCRGTCAAACTAAGCAGCGGYTAYAA-BHQ1 | 1538-1565 | |||
N9 | N9 forward | GCCCTGATAAGCTGGCCACT | 472-491 | ||
N9 reverse | ACTAGTACTTGACCAMCCAATGCA | Neuraminidase | 529-552 | KC885958.1 | |
N9 probe | HEX- TCACCRCCCACAGTRTACAAYAGCA-BHQ1 | 496-520 | |||
RP | RP forward | AGATTTGGACCTGCGAGCG | 50-68 | ||
RP reverse | GAGCGGCTGTCTCCACAAGT | Ribonuclease P | 71-93 | NM_006413.4 | |
RP probe | CY5- TTCTGACCTGAAGGCTCTGCGCG-BHQ2 | 95-114 | |||
Designed in the World Health Organization (WHO) protocol and used in this study | |||||
FluA | InfA Forward | GACCRATCCTGTCACCTCTGAC | 146-167 | ||
InfA Reverse | AGGGCATTYTGGACAAAKCGTCTA | Matrix protein | 228-251 | ||
InfA Probe | FAM-TGCAGTCCTCGCTCACTGGGCACG-BHQ1 | 201-224 | |||
H7 | CNIC-H7F | GGTTTTTTCTTGTATTTTTATATGACTTAG | 468-490 | ||
CNIC-H7R | GGTTTTTTCTTGTATTTTTATATGACTTAG | Hemagglutinin | 521-550 | ||
CNIC-H7P | FAM-AGATAATGCTGCATTCCCGCAGATG-BHQ1 | 495-519 | |||
N9 | CNIC-N9F | TGGCAATGACACACACTAGTCAGT | 929-952 | ||
CNIC-N9R | ATTACCTGGATAAGGGTCGTTACACT | Neuraminidase | 1010-1035 | ||
CNIC-N9P | FAM- AGACAATCCCCGACCGAATGACCC -BHQ1 | 975-998 | |||
Rnase P | Rnase P Forward | AGATTTGGACCTGCGAGCG | 50-68 | ||
Rnase P Reverse | GAGCGGCTGTCTCCACAAGT | Ribonuclease P | 71-93 | ||
Rnase P Probe | FAM-TTCTGACCTGAAGGCTCTGCGCG-BHQ1 | 95-114 | |||