Differential in vivo expression of mycobacterial antigens in Mycobacterium tuberculosisinfected lungs and lymph node tissues

Table 3 Immunohistochemical analysis of in vivo expression of various mycobacterial antigens in the formalin fixed tissues obtained from Mycobacterium tuberculosis -infected lungs and lymph nodes, and non-tuberculous controls

	No. with positive results/total number (%)***
	Multibacillary lung tissues**	Tuberculous lymphadenitis	Non-TB controls
ZN staining	3/3 (100)	0/17 (0)	0/12 (0)
PCR*	3/3 (100)	17/17 (100)	0/12 (0)
*Secreted antigens*
MPT32	3/3 (100)	3/17 (18)	0/12 (0)
MPT44	1/3 (33)	4/14 (28)	6/12 (50)
MPT46	3/3 (100)	0/17 (0)	1/12 (8)
MPT51	3/3 (100)	0/17 (0)	0/12 (0)
MPT53	3/3 (100)	3/17 (18)	1/12 (8)
MPT59	3/3 (100)	6/17 (35)	3/12 (25)
MPT63	3/3 (100)	2/17 (12)	2/12 (17)
MPT64	3/3 (100)	17/17 (100)	2/12 (17)
*Somatic antigens*
Mce1A	3/3 (100)	17/17 (100)	8/12 (67)
Hsp65	3/3 (100)	17/17 (100)	12/12 (100)
MPT57	3/3 (100)	16/16 (100)	12/12 (100)

*PCR, N-PCR for amplification of IS6110.
**Vast number of bacilli were detectable by ZN staining.
***A case/control was labelled as positive if any positive signal was recorded in a lesion irrespective of the quality of stain.

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