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Table 2 Antibodies used in the immunohistochemical staining

From: Differential in vivo expression of mycobacterial antigens in Mycobacterium tuberculosisinfected lungs and lymph node tissues

Antibody Target antigen Protein function Dilution
Secreted antigens    
Anti-MPT32 MPT32 (Apa, Rv1860) Unknown (Could mediate bacterial attachment to host cells) 1:500
Anti-MPT44 MPT44 (Ag85A, fbpA, Rv3804c) Involved in cell wall mycoloylation. Responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. 1:100
Anti-MPT46 MPT46 (Thioredoxin TrxC, Rv3914) Participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions. 1:500
Anti-MPT51 MPT51 (Ag85D, fbpD, Rv3803c)/TB22.2 Same as MPT44 1:500
Anti-MPT53 MPT53 (DsbE, Rv2878c) Unknown 1:500
Anti-MPT59 MPT59 (Ag85B, fbpB, Rv1886c) Same as MPT44 1:100
Anti-MPT63 MPT63 (Rv1926c) Unknown 1:500
Anti-MPT64 MPT64 (Rv1980c) Unknown (Suggested to inhibit apoptosis of infected cells) 1:250
Somatic antigens    
Anti-Mce1A Mce1A (Rv0169) Thought to be involved in cell invasion (entry and survival) 1:2000
Anti-Hsp65 Hsp65 (Ag82, GroEL2, Rv0440) Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions. 1:500
Anti-MPT57 MPT57 (GroES, Rv3418c) Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. 1:4000