Table 2 Antibodies used in the immunohistochemical staining

Secreted antigens

Anti-MPT32

MPT32 (Apa, Rv1860)

Unknown (Could mediate bacterial attachment to host cells)

1:500

Anti-MPT44

MPT44 (Ag85A, fbpA, Rv3804c)

Involved in cell wall mycoloylation. Responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity.

1:100

Anti-MPT46

MPT46 (Thioredoxin TrxC, Rv3914)

Participates in various redox reactions through the reversible oxidation of its active center dithiol, to a disulfide, & catalyzes dithiol-disulfide exchange reactions.

1:500

Anti-MPT51

MPT51 (Ag85D, fbpD, Rv3803c)/TB22.2

Same as MPT44

1:500

Anti-MPT53

MPT53 (DsbE, Rv2878c)

Unknown

1:500

Anti-MPT59

MPT59 (Ag85B, fbpB, Rv1886c)

Same as MPT44

1:100

Anti-MPT63

MPT63 (Rv1926c)

Unknown

1:500

Anti-MPT64

MPT64 (Rv1980c)

Unknown (Suggested to inhibit apoptosis of infected cells)

1:250

Somatic antigens

Anti-Mce1A

Mce1A (Rv0169)

Thought to be involved in cell invasion (entry and survival)

1:2000

Anti-Hsp65

Hsp65 (Ag82, GroEL2, Rv0440)

Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.

1:500

Anti-MPT57

MPT57 (GroES, Rv3418c)

Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.

1:4000