Figure 4

The RXR antagonist HX531 reduced the viability of adult T. muris in vitro . Worms were extracted from mice at day 40 post-infection and incubated for 24 h with vehicle, the anthelmintics mebendazole and pyrantel embonate, the RXR agonist HX630, the RXR antagonist HX531 or bleach. Worm viability was assessed using (A) a motility score, in which 0 = dead, 1 = very low motility, 2 = low motility and 3 = normal motility, or (B) MTT assay. An Andor camera was used to capture the movement of T. muris by filming for 20s. Pixel changes from frame to frame were captured as an indicator or motility (C) and quantified using Matlab software (D). Data are representative of two independent experiments. (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, compared to controls, unless denoted otherwise.