Activation of TLR2 in response to the SMG. A. HEK293 cells expressing TLR2 and an NF-κB:SEAP reporter were stimulated with heat-killed SMG from the low (n = 15), intermediate (n = 6), or high (n = 10) cytokine response groups at a ratio of 500:1 bacteria:HEK293 cells. Detection of SEAP expression was determined by measuring absorbance at 630 nm after 24 hours. Circles represent the relative TLR2 induction by a single isolate. Data are the mean of three replicates. Statistical significance was determined by Kruskal-Wallis test with Dunn’s post hoc comparison. Ns, not significant. B. Reporter cells were stimulated with serial dilutions of the TLR2 ligand Pam3CK4 for 24 hrs and OD630 was measured.