Cytokine responses to clinical isolates of the SMG. PBMCs were stimulated with 35 heat-killed isolates of the SMG (12 S. anginosus, 11 S. constellatus, and 12 S. intermedius) at a ratio of 5:1 (PBMCs:bacteria). Levels of IL1β, TNF, IL10, IL23, 12p70, IL17A and IL4 were measured in cell supernatant after 24 hours by multiplex assaying. A-G. Box-and-whisker plots indicate median, interquartile ranges and outliers of cytokine production in response to isolates of each species. S. ang = S. anginosus, S. const = S. constellatus, S. int = S. intermedius. Statistical significance was determined by Kruskal-Wallis test with Dunn’s post hoc comparison. ** P < 0.01.