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Table 2 Positive cytokine responses per antigen before and after 2 weeks of TB treatment

From: Cytokine response to selected MTB antigens in Ghanaian TB patients, before and at 2 weeks of anti-TB therapy is characterized by high expression of IFN-γ and Granzyme B and inter- individual variation

  No. of positive responders per antigen [n1/N2(%)]
SEB E6/C10 Rv1733 Rv2029 Rv2628
Granzyme B      
Before 19/19 (100) 13/19 (68.4) 13/18 (72.2) 7/11 (63.6) 7/10 (70)
After 2wks 14/15 (93.3) 12/15 (80.0) 9/10 (90.0) 7/7 (100.0) 5/5 (100)
P value 0.4412 0.6974 0.3746 0.1193 0.5055
IFN-γ      
Before 18/19 (94.7) 16/19 (84.2) 15/18 (83.3) 8/11 (72.7) 8/10 (80.0)
After 2wks 12/15 (80.0) 13/15 (86.6) 10/10 (100) 7/7 (100) 5/5 (100)
P value 0.2994 1.0 0.5330 0.2451 0.5238
IL-10      
Before 18/19 (94.7) 14/19 (73.7) 12/18 (66.7) 7/11 (63.6) 5/10 (50.0)
After 2wks 12/15 (80) 9/15 (60.0) 7/10 (70.0) 4/7 (57.1) 5/5 (100)
P value 0.2994 0.4748 1.0 1.0 0.1009
IL-17      
Before 19/19 (100) 13/19 (68.4) 14/18 (77.8) 6/11 (54.5) 8/10 (80.0)
After 2wks 13/15 (86.6) 12/15 (80.0) 9/10 (90.0) 6/7 (85.7) 5/5 (100)
P value 0.1872 0.6974 0.6264 0.3156 0.5238
sIL-2R-α      
Before 18/19 (94.7) 14/20 (70.0) 12/18 (66.7) 7/11 (63.6) 8/10 (80.0)
After 2wks 11/15 (73.3) 12/15 (80.0) 9/10 (90.0) 6/7 (85.6) 5/5 (100)
P value 0.1458 0.7003 0.3642 0.5956 0.5238
TNF-α      
Before 16/19 (84.2) 13/19 (68.4) 12/18 (66.7) 7/11 (63.6) 7/10 (70.0)
After 2wks 11/15 (73.3) 9/10 (90.0) 9/10 (90.0) 5/7 (71.4) 5/5 (100)
P value 0.6722 0.3667 0.3642 1.0 0.5055
  1. Positive cytokine responses per antigen at baseline and 2 weeks of anti-TB therapy. Freshly isolated PBMC from Sputum smear positive TB patients were stimulated for 6 days before initiation of therapy (baseline) with SEB (n = 19), ESAT-6/CFP-10 (n = 19), Rv1733 (n = 18), Rv2029 (n = 11) and Rv2628 (n = 10) and after 2 weeks on anti-TB therapy with SEB (n = 15), ESAT-6/CFP-10 (n = 15), Rv1733 (n = 10), Rv2029 (n = 7) and Rv2628 (n = 5). Culture supernatant was assessed by multiplex cytokine analysis. Percentage of positive cytokine (Granzyme B, IFN-γ, IL-10, IL-17, sIL2R-α and TNF-α) responses per antigenic stimulation were calculated. Positive responses were determined as follows; Cytokine concentrations were divided by the negative control sample (medium only, un-stimulated) and values greater than or equal to 2 were considered positive.
  2. 1Number of positive responses.
  3. 2Number of samples analyzed.