High level of IFN-γ and Granzyme B are secreted in response to all antigens. Freshly isolated PBMC obtained from sputum smear positive TB patients (n = 19) at baseline (before treatment) were stimulated in vitro with the stage specific M. tuberculosis antigens; ESAT-6/CFP-10, Rv1733, Rv2029, Rv2628 (latency associated), positive control Staphylococcus enterotoxin B (SEB) and negative control (Growth medium) for 6 days. The harvested supernatant were used in a six-plex Luminex assay for of Granzyme B (GrzB), IFN-γ, IL-10, IL-17, sIL2Rα and TNF-α. The box plots show the 25th, 50th, and 75th percentiles, and the whiskers represent the minimum and maximum levels of cytokine (pg/ml) induced by each stimulus. In response to all antigens, high levels of IFN-γ followed by Granzyme B and TNF-α and low levels of IL-17, sIL2Rα and IL-10 were observed.