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Table 1 Cytokine production by splenocytes of immunized Kunming mice after stimulation by Toxoplasma lysate antigen (TLA)

From: Protective efficacy of Toxoplasma gondiicalcium-dependent protein kinase 1 (TgCDPK1) adjuvated with recombinant IL-15 and IL-21 against experimental toxoplasmosis in mice

Group (n = 3)

Cytokine production (pg/ml)

Proliferation (SI)

 

IFN-γ

IL-2

IL-4

IL-10

 

pVAX-CDPK1 + pVAX-IL-21-IL-15

867.78 ± 15.63A*

532.54 ± 23.06A

154.44 ± 11.16A

131.04 ± 10.31A

5.57 ± 0.01A

pVAX-IL-21-IL-15

505.12 ± 32.72B

314.77 ± 27.06B

105.15 ± 16.82B

96.54 ± 20.03B

3.79 ± 0.05B

pVAX-CDPK1

563.65 ± 12.97C

353.12 ± 10.93C

111.94 ± 8.90B

109.72 ± 8.65B

4.19 ± 0.14C

pVAX I

53.02 ± 3.73D

52.04 ± 1.43D

51.01 ± 1.08C

51.13 ± 1.70C

1.05 ± 0.00D

PBS

52.92 ± 2.73D

51.49 ± 2.67D

49.84 ± 1.37C

50.20 ± 1.24C

1.04 ± 0.07D

Control

52.53 ± 2.43D

51.56 ± 1.43D

49.50 ± 1.79C

49.75 ± 1.16C

1.03 ± 0.03D

  1. SI stimulation index.
  2. Splenocytes from 3 mice were harvested 2 weeks after the last immunization.
  3. Values for IFN-γ are after 96 h, values for IL-2 and IL-4 are after 24 h, and values for IL-10 are after 72 h.
  4. *The same superscript letter “D” on the shoulders of experimental data means no statistically significant difference (P > 0.05) among different experimental groups from the same measurement, while different letter including A, B, C means statistically significant difference among different experimental groups from the same measurement (P < 0.05).