complex detection through the amplification if the MPB64 immunogenic protein. The DNA Marker shows two bands which are the internal control band (520 bp) and the M. tuberculosis band at (190 bp). This shows how positive samples should be. The positive control shows two bands as well, the internal control (520 bp) and an upper band corresponding to 745 bp (instead of 190 bp) which is designed by the manufacturer to eliminate false positive resulting from cross contamination. The negative control shows only the internal control (520 bp), negative samples shows only this band. Lane 1: DNA Marker; lane 2: Positive control; lane 3–7 DNA samples; lane 8: Negative control.