Minimum spanning tree showing the MLST pattern of 434 Chlamydia trachomatis positive samples from 206 women with concurrent infections at multiple anatomic locations. Each circle represents one MLST type. Size of the circles is proportional to the number of identical STs. Bold lines connect types that differ for one single locus. Halos indicate the distinct clusters. White indicates concordant samples (maximum 1 locus difference in MLST profile between infections) within one woman. Colors indicate discordant samples, with a unique color for each of the 27 women. Discordant is more than 1 locus difference in MLST profile between infections.