Minimum spanning tree showing the MLST pattern of 434 Chlamydia trachomatis positive samples from 206 women with concurrent infections at multiple anatomic locations. Each circle represents one MLST type. Size of the circles is proportional to the number of identical STs. Bold lines connect types that differ for one single locus. Halos indicate the distinct clusters. Colors indicate the anatomic location of sampling; pink: cervical samples (n = 149), cyan: urethral samples (n = 116), orange: vaginal samples (n = 51), blue: anorectal samples (n = 101), and yellow: pharyngeal samples (n = 17).