Figure 2

Phenotypic analysis of Ly5.1 ⁺ cells that migrated from the peritoneum to the LN after Leishmania infection. Infected (L. amazonensis) or control (medium alone) cells from C57BL/6 Ly5.1⁺ mice were injected i.p. into C57BL/6 Ly5.1^- mice previously stimulated with thioglycollate. After 24 hours, the LNs were collected and only Ly5.1⁺ cells were analyzed. The main phagocyte populations are defined by the expression of the indicated markers. (A) Dot plots from one representative experiment with values representing the mean ± sd of three experiments that were independently performed. (B) Comparison of the proportion of migrating phagocytes of different phenotypes from the inflammatory site to the draining LN after incubation with medium alone (control) or with L. amazonensis (infected) (10 promastigotes per PEC). Each point represents the percentage of cells among the migrating leukocytes (injected into the peritoneal cavity and tracked to the LN) relative to the percentage of the same injected cell population remaining in the peritoneal cavity.