Phenotypic analysis of the
cell transfer-and-tracking assay from C57BL/6 mice to BALB/c mice. Infected (L. amazonensis) or control (medium alone) thioglycollate-elicited peritoneal exudate cells (PEC) of C57BL/6 mice were injected i.p. in BALB/c mice previously stimulated with thioglycollate. After 24 hours, the PEC and draining thymic LNs were collected, and the H-2Kb+ (C57BL/6) cells were analyzed. The main phagocyte populations were defined by the expression of the indicated markers. (A) Dot plots from one representative experiment with values representing the mean ± sd of four experiments that were independently performed. (B) Comparison of the proportion of migrating phagocytes of different phenotypes from the inflammatory site to the draining LN after incubation with medium alone (control) or with L. amazonensis (infected) (10 promastigotes per PEC). Each point represents the percentage of cells among the migrating leukocytes (injected into the peritoneal cavity and tracked to the LN) relative to the percentage of the same injected cell population remaining in the peritoneal cavity.