Standard curve obtained with the G6PD
qPCR test. The standard curve was generated by plotting the Ct against the input target quantity (in this case, serial dilutions of Leishmania DNA respresenting 106 to 101 parasites). Ct represents the fractional cycle number reflecting a positive PCR result differentiated from the background noise. Slope -3.206, PCR efficacy 105%, R2 = 0.971.