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Table 1 Primers and probes used for qPCR

From: Persistence, clearance and reinfection regarding six high risk human papillomavirus types in Colombian women: a follow-up study

Region

Viral type

Primer sequence 5′ - 3′

mT*

Plasmid product quantification

qPCR test

Probe

Probe size (bp)

Quencher

E7

HPV-16

AGCTCAGAGGAGGAGGAT

54

1.43E1012

Reaction 1

FAM

78

ZEN/Iowa Black FQ

  

GGTTACAATATTGTAATGGGCTC

      

E1

HPV-18

CATTTTGTGAACAGGCAGAGC

53.7

1.19E1012

Reaction 2

Cy5

80

IBRQ

  

ACTTGTGCATCATTGTGGACC

      

E6

HPV-31

ACGATTCCACAACATAGGAGGA

53.7

1.35E1012

 

HEX

78

ZEN/lowa Black FQ

  

TACACTTGGGTTTCAGTACGAGGT

      

E7

HPV-33

ATTAAGTGACAGCTCAGATGA

53.7

1.86E1012

Reaction 3

FAM

78

ZEN/Iowa Black FQ

  

ACATAAACGAACTGTGGTGTT

      

E1

HPV-45

CCATTTGTGAACAGGCAGAGC

53.7

1.59E1012

 

Cy5

76

IBRQ

  

CAACACCTGTGCATCATTCTGA

      

E7

HPV-58

CGAGGATGAAATAGGCTTGG

53.7

1.23E1012

Reaction 4

HEX

109

ZEN/Iowa Black FQ

  

ACACAAACGAACCGTGGCGT

      

HMBS

GCCTGCAGTTTGAAATCAGTG

53.7

1.98E1012

 

FAM

76

ZEN/Iowa Black FQ

  

CGGGACGGGCTTTAGCTA

      
  1. HPV Human papillomavirus, mT Melting temperature in °C, FAM 6-carboxyfluorescein, Cy5 FluoroLink Mono Reactive Dye Cy5, HEX Hexachlorofluoresceine, HMBS, Hydroxymethylbilane synthase.
  2. *Melting temperature for quantitative real-time PCR.